The comet assay is a simple method for measuring dna strand breaks in eukaryotic cells. Our cometassay product line is the only complete and optimized commercially available platform for the comet assay, which include reagents, kits, equipment and analysis software. It can usually be performed on materials found in most laboratories. In vivo alkaline comet assay and enzymemodified alkaline. The comet analysis software can rapidly evaluate large numbers of cells and generate summary statistics, increasing processivity approximately 10,000% over traditional comet analysis. The trevigen team is here to assist you with your research needs. The comet assay, also known as single cell gel electrophoresis scre, helps scientists to determine whether there has been deoxyribonucleic acid dna damage to a. Bleomycin treatment t3 1 800 trevigen bleomycin healthy e 30 10 bleomycin alkaline comet hydrogen peroxide healthy. The control untreated cells should be used to determine the. This was a neutral version of the comet assay, and interestingly, they used quite sophisticated. The single cell gel electrophoresis assay, also known as the comet assay, is a. Horizontal electrophoresis chamber, trevigen, cometassay es ii.
From engineering to manufacturing to sales and support, biotek employees. The comet assay can distinguish small differences in dna damage between different samples of cells, implying that statistical tests are important to assess whether this occurs by chance. Opencomet is a free software tool for measuring dna damage in comet assay images. For decades, researchers have measured dna damage using the single cell gel. Whether you are looking for custom packaging, different size, or even something other than our product offerings, we will work with you. The availability of powerful automated and free comet assay imaging software is currently one of the major limitations in using the assay. This entry was posted in casplab news on december 11, 20 by. A highthroughput 96well platform for measuring dna. The software applies state of the art image processing methods to automatically locate scorable. Metafer 1 is the perfect system for automated, highthroughput comet assay analyses. It is expensive but give good results in respect to quantitation. The comet assay is a wellpublished method for measuring cellular dna damage. Yet, i have not had good results of a reliable software. Combining a novel comet assay procedure with automated.
Ams biotechnology europe limited, 184 park drive, milton park, abingdon ox14 4se, uk. Casplab comet assay software project lab is a crossplatform image analysis software to measure level of dna damage in scge single cell gel casplab comet assay software project browse casp1. Opencomet helps to overcome the current limitations. The cometchip is now commercially available through trevigen. Comet assay as an indirect measure of systemic oxidative. Comet assay iv combines accurate, repeatable results with powerful data processing and comprehensive. Trevigen has developed a set of suspension cell preparations containing different levels of dna damage to be used as controls with trevigen s cometassay kits. Comet assay does not require any special apparatus or chemicals. During electrophoresis, smaller, damaged dna fragments travel. The comet assay was first introduced by ostling and johanson in 1984. Casplab is a tool to image analysis in comet assay. Trevigen s comet analysis software can rapidly evaluate large numbers of cells, related to each treatment group or screening target in a study, and generate summary statistics based on the corresponding numeric results. A cross platform public domain pc image analysis program for the comet assay.
Sources of variability in the comet assay include variations in the protocol used to process the cells, the microscope imaging system and the software used in the computerized. Comet assay single cell gel electrophoresis sigmaaldrich. The comet assay is an efficient method to detect dna damage including single. Tech is a leading developer and manufacturer of high quality purified proteinsnotably cytokines and growth factors, antibodies, immunoassays, as well as. Cometslides allow direct application of comet lmagarose without base layers or pretreatment. The software applies state of the image processing methods to automatically locate and score comets to characterize and quantify the degree of dna damage. Comet assay and foci dna damage quantification metasystems. The software applies state of the image processing methods to automatically locate and score comets to characterize and quantify the degree of dna damage revealed by the comet assay.
Department of biomedical engineering, university of minnesota. In this study, we present a semiautomatic comet assay analysis tool that was developed in our laboratory. Which fluorescent dna stain is the best for comet assay. Evaluating in vitro dna damage using comet assay protocol jove. The single cell gel electrophoresis assay scge, also known as comet assay is an uncomplicated and sensitive technique for the detection of dna damage at the level of the individual. The ability to quantify dna damage responses is thus critical in the. The alkaline comet assay has many applications in genetic toxicology and human biomonitoring. The comet assay is a common follow up to a positive in vitro assay such as the chromosome aberration, mouse lymphoma andor invitro micronucleus assay. Jack chirkjian and michael elliot and is located in gaithersburg, maryland.
Biology cancer research chemistry developmental biology engineering. Trevigen has a wide range of products for measuring oxidative stress and dna damage. The comet assay, also known as singlecell gel electrophoresis scge. Automated imaging and analysis of a novel comet assay to. Trevigen comet analysis software training video youtube. The results obtained with our tool were compared with that of fully automatic.
When performing alkaline electrophoresis, the four control cell. Data can be exported and analyzed using statistic tools. Casplab comet assay software project lab is a crossplatform image analysis software to measure level of. Influenza infection induces host dna damage and dynamic dna damage responses during tissue regeneration. Analyze comet images by standard comet assay software such as komet 5. Through integration of engineering technologies and principles, the cometchip brings a. The flare fragment length analysis using repair enzymes assay provides the ability to probe for oxidative dna damage in conjunction with cometassay reagents. The cometchip is a consumable consisting of specifically sized micron pores patterned into agarose.
In addition, inhouse software enables automated comet analysis, which reduces bias and increases throughput. Hydrophobic barrier allows easy application of cells directly to slide, and. Micropatterned comet assay enables high throughput and sensitive. Comet assay was first developed by swedish scientists ostling and johanson 1. Micropatterned comet assay enables high throughput and sensitive dna damage quantification. The cometchip electrophoresis starter kit which includes one cometchip reagent kit, the 96well cometchip system macrowell former, and the cometassay electrophoresis system ii for consistent and reproducible results. The comet tail can be scored according to dna content intensity. We validated opencomet on neutral comet assay images, which present different characteristics from alkaline comet assay images. Under alkaline conditions ph, the comet assay can detect single and double stranded breaks.
The comet assay, in specific, is widely used for measuring oxidative and other types of dna damage. Trevigen s comet analysis software provides the computational power to efficiently analyze digital images representing fields of cells run in the comet assay. Casplab has been developed to work with either color, or grayscale images of fluorescencestained comets saved in tif format. The cometassays cometslide is specially treated to promote adherence of low melting point.
The comet assay is a gel electrophoresis method used to visualize and measure. The purpose of the comet assay is to identify substances that cause dna damage. The comet assay is well established for quantifying dna damage in. Standard alkaline comet assay slides were prepared as follows or. Comet assay iv scoring software in order to quickly and accurately score cells using live video imaging, you will need the comet assay iv scoring software and camera. Dna fragmentation associated with oxidative dna damage was. Our comet assays provide a fast, convenient way to screen for general dna damage, regardless of the. Comprehensive measurements and results easily graph and report your data.
In fact, the alkaline comet assay is now often the method of choice if low levels of dna damage are to be detected, e. It is specially engineered to complement and operate as the computational component of trevigen s unique cometassay electrophoresis system. Standard alkaline comet assay slides were prepared as follows or obtained directly from trevigen. Automated imaging and analysis of a novel comet assay to enable. We have validated opencomet on both alkaline and neutral comet assay images as well as sample. I have been searching for the best software to acquire more variables from the comet assay. Breakthrough software with unmatched performace, designed to improve, standardize, and make comet analysis easier.
The single cell gel electrophoresis scge or comet assay is a sensitive and rapid technique for quantifying dna damage and repair from in vivo and in vitro samples of eukaryotic and plant. Trevigen was founded in 1992 by scientists and entrepreneurs drs. Cells embedded in agarose on a microscope slide are lysed with detergent and high salt to form. Undamaged cells had the appearance of the comet tail moment tm analyzer as comet assay parameter and image should analyze using comet image analyzer and data. The standard alkaline comet assay procedure was performed using alkaline. The in vitro and in vivo comet assays springerlink. Trevigens comet analysis software provides the computational power. The comet assay, also called single cell gel electrophoresis scge, is a sensitive and rapid technique for quantifying and analyzing dna damage in individual cells. Opencomet is an opensource software tool providing automated analysis of comet assay images. After 10 min of gelling at 4 c, the cells were lysed with lysis solution trevigen, usa at 4 c for 1 h.
Our oxiselect 96well comet assay kits provide a higherthroughput way to screen for general dna damage, regardless of the source or nature of the damage. Comet assay results, measurements and data processing. Download casplab comet assay software project for free. Trevigen comet analysis software is a platform that is capable of evaluating a large number of cells as well as generating its corresponding summary. The 96well cometchip system is a highthroughput platform to simultaneously treat and measure dna damage induced by different treatments, or among different cell types on a single slide using the comet assay.
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